LOCUS       sequence-50381-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  TITLE     Glick Lab Modified Fluorescent Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmECFP-1
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..229
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    282..359
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(565..584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
       61 agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      121 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      181 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      241 ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga tcccgggccc
      301 gcggtaccgt cgactgcaga attcgaagct tgagctcgag atctgagtcc ggtagcgcta
      361 gtaataacta atgcatggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      661 gaagcgtggc gctttctcat agctcacgct gtaggtatct ca
//
