LOCUS       sequence-50382-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 1571
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..100)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      253..339
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      431..458
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    551..567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(664..683)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 atcaagcagc aaacgacgaa aactacgctg cagcagttta atgctagctt ggctgttttg
       61 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
      121 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
      181 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
      241 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      301 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      361 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      421 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
      481 tttatttttc taaatacatt caaatatgta tccgctcagg atccataaat gtgagcggat
      541 aacattgaca ttgtgagcgg ataacaagat actcccgggt aaggaggaaa atgtctagat
      601 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      661 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      721 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      781 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgct
//
