LOCUS       sequence-50387-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL600
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    245..261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(316..402)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    801..819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    826..844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccctgtacga ttaaagcttc tttcaggtac cttagtaact ggactgctgg gatttttcag
       61 cctggatacg ctggtagatc tcttcacggt gaacagaaac ttccttcggg gcatttacgc
      121 caatacgtac ctggttgccc tttaccccta aaactgtcac ggtgacctca tccccaatca
      181 tgagggtctc accaactcga cgagtcagaa tcagcatttt ttttcctcct tacccgggag
      241 tatcttgtta tccgctcaca atgtcaatgt tatccgctca catttatgga tccgcgccta
      301 ggtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      361 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctct agcacgcgta
      421 ccatgggccc aataaaaaaa gggagcactg tattcacagc gctcccggtt cgtttcgcag
      481 cattccagct acttttgttg ctccctgctc atccttgaca acttttcctc tggccttgcg
      541 gccaatcgtt catcctgaac tattgcttcc tgctcacacc accccgatgt gatacttcat
      601 cctgaagtgt ccctggcctt cctgacccac cgaatcatcc tgaccggttc tcattctcca
      661 tcctggaggt gtcctttaac gcgtcctgcg tcatcctctt cgcttcatcc agaagccttt
      721 ccctgaaaca ccatcctggt gtgtcctgca gaagtgtcat catcctgatg ttcacttcgt
      781 tgtctgactc gtcgacagta tctctatcac tgatagggat gtcaatctct atcactgata
      841 gggactcgag
//
