LOCUS       sequence-50410-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Zou J, Mali P, Huang X, Dowey SN, Cheng L
  TITLE     Site-specific gene correction of a point mutation in human iPS cells
            derived from an adult patient with sickle cell disease.
  JOURNAL   Blood. 2011 Oct 27;118(17):4599-608. Epub 2011 Aug 31.
  PUBMED    21881051
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood."; 
            date: "2011-10-27"; volume: "118(17)"; pages: "4599-608. Epub 2011 
            Aug 31"
COMMENT     Sequence Label: BD2
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..46
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccggtagaat tcataacttc gtataatgta tgctatacga agttatgcta gcggtctcct
       61 taaacctgtc ttgtaacctt gataccaacc tgcccagggc ctcaccacca acttcatcca
      121 cgttcacctt gccccacagg gcagtaacgg cagacttctc ctcaggagtc aggtgcacca
      181 tggtgtctgt ttgaggttgc tagtgaacac agttgtgtca gaagcaaatg taagcaatag
      241 atggctctgc cctgactttt atgcccagcc ctggctcctg ccctccctgc tcctgggagt
      301 agattggcca accctagggt gtggctccac agggtgaggt ctaagtgatg acagccgtac
      361 ctgtccttgg ctcttctggc actggcttag gagttggact tcaaaccctc agccctccct
      421 ctaagatata tctcttggcc ccataccatc agtacaaatt gctactaaaa acatcctcct
      481 ttgcaagtgt atttacgtaa tatttggaat cacagcttgg taagcatatt gaagatcgtt
      541 ttcccaattt tcttattaca caaataagaa attgatgcac taaaagtgga agagttttgt
      601 ctaccataat tcagctttgg gatatgtaga tggatctctt cctgcgtctc cagaatatgc
      661 aaaatactta caggacagaa tggatgaaaa c
//
