LOCUS       sequence-50411-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Pellegrin S, Mellor H
  TITLE     The Rho family GTPase Rif induces filopodia through mDia2.
  JOURNAL   Curr Biol. 2005 Jan 26;15(2):129-33.
  PUBMED    15668168
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2005-01-26"; volume: "15(2)"; pages: "129-33"
COMMENT     Sequence Label: myc Rif wild-type
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..127
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(798..814)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(798..814)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttgatagct taccatggaa caaaaactaa tatcggaaga
      121 agatctaggg cccatggatg cccccggagc cctggcccag accgccgccc ccggtccggg
      181 caggaaggag ctgaagatcg tgatcgtggg cgacggcggc tgcggcaaga cctcgctgct
      241 catggtgtac agccagggct ccttccccga gcactacgcc ccatcggtgt tcgagaagta
      301 cacggccagc gtgaccgttg gcagcaagga ggtgaccctg aacctctacg acacggccgg
      361 gcaagaagac tatgaccggc tgcggcccct gtcctaccag aacacccacc tcgtgctcat
      421 ctgctatgac gtcatgaatc ccaccagcta cgacaacgtc ctcatcaagt ggttccctga
      481 ggtcacgcat ttctgccgcg ggatccccat ggtgctcatc ggctgcaaga cagacctgag
      541 gaaggacaag gagcagctgc ggaagctccg ggccgcccag ctggagccca tcacctacat
      601 gcagggcctg agcgcctgcg aacagatccg agctgctctc tacctggaat gttccgccaa
      661 gtttcgggag aatgtggagg acgtcttccg ggaggccgcc aaggtggctc tcagcgctct
      721 gaagaaggcg caacggcaga agaagcgccg gctctgcctg ctgctctgat ctagaatcga
      781 attcctgcag cccgggggat ccactagttc tagagggccg tattctatag tgtcaccta
//
