LOCUS       sequence-50414-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Pellegrin S, Mellor H
  TITLE     The Rho family GTPase Rif induces filopodia through mDia2.
  JOURNAL   Curr Biol. 2005 Jan 26;15(2):129-33.
  PUBMED    15668168
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2005-01-26"; volume: "15(2)"; pages: "129-33"
COMMENT     Sequence Label: myc Rif-TN
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(795..811)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(795..811)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(821..839)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(822..839)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(859..876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgatagctta ccatggaaca aaaactaata tcggaagaag
      121 atctagggcc catggatgcc cccggagccc tggcccagac cgccgccccc ggtccgggca
      181 ggaaggagct gaagatcgtg atcgtgggcg acggcggctg cggcaagaac tcgctgctca
      241 tggtgtacag ccagggctcc ttccccgagc actacgcccc atcggtgttc gagaagtaca
      301 cggccagcgt gaccgttggc agcaaggagg tgaccctgaa cctctacgac acggccgggc
      361 aagaagacta tgaccggctg cggcccctgt cctaccagaa cacccacctc gtgctcatct
      421 gctatgacgt catgaatccc accagctacg acaacgtcct catcaagtgg ttccctgagg
      481 tcacgcattt ctgccgcggg atccccatgg tgctcatcgg ctgcaagaca gacctgagga
      541 aggacaagga gcagctgcgg aagctccggg ccgcccagct ggagcccatc acctacatgc
      601 agggcctgag cgcctgcgaa cagatccgag ctgctctcta cctggaatgt tccgccaagt
      661 ttcgggagaa tgtggaggac gtcttccggg aggccgccaa ggtggctctc agcgctctga
      721 agaaggcgca acggcagaag aagcgccggc tctgcctgct gctctgatct agatcgaatt
      781 cctgcagccc gggggatcca ctagttctag agggccgtat tctatagtgt cacctaaatg
      841 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg c
//
