LOCUS       sequence-50415-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N BALF1
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..45
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             58..84
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggaattagat ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac
       61 ccatacgatg ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagtt
      121 ggcatgaggc cagccaagtc tacagattct gtgtttgtga ggaccccggt cgaggcgtgg
      181 gtcgcgccct cgccgccgga cgacaaggtg gctgagtcca gctacctcat gttcagggcc
      241 atgtacgcgg tgttcacccg ggatgagaaa gacctgcctt tgccagccct ggtcctctgc
      301 cggctcatca aggcctccct gaggaaggat aggaagctgt acgcggagct ggcctgcagg
      361 acagccgaca tcgggggcaa agacacgcac gtacggctca tcatcagcgt cctgcgcgca
      421 gtgtacaacg accactacga ctactggtcg cggctcaggg tggtgctgtg ctacacagtg
      481 gtgtttgcgg tgcgaaacta cctggatgac cacaagagcg ccgccttcgt gctgggggca
      541 atcgcccact acctggccct ctatcgcaga ctctggtttg cgaggctggg cggcatgcca
      601 agatcgctga g
//
