LOCUS       sequence-50422-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     Callaway lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-HB
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(30..407)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     protein_bind    423..456
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    507..540
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(618..638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ttgctcacca tggtggcgac cggtggatcc ttagcgctgg tgtacttggt gatggcctta
       61 gtaccctcgg acacggcgtg cttggccaac tccccaggca gcagcaggcg cacggccgtc
      121 tggatctccc tggaggtgat ggtcgagcgc ttgttgtaat gcgccaggcg ggaagcctca
      181 cctgcgatgc gctcgaaaat gtcgttcaca aacgaattca tgatgcccat ggccttggac
      241 gaaatgccgg tgtcagggtg gacctgcttc agaaccttgt acacatagat ggaatagctc
      301 tccttgcggc tgcgcttgcg cttcttgccg cctttcttct gcgccttagt caccgccttc
      361 ttggagccct ttttcggggc gggagcagac ttcgctggct ctggcatggt ggcggctcta
      421 gcataacttc gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac
      481 tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat acgaagttat
      541 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
      601 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
      661 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
      721 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
      781 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
      841 gggactttcg ctttccccct cc
//
