LOCUS       sequence-50424-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: pBad-Int Set Flipper
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(582..601)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(666..685)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
       61 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
      121 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
      181 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt
      241 aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc
      301 ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag
      361 agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc
      421 tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca
      481 acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      541 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      601 tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt
      661 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
      721 gtatgggaag cccgatgcgc cagagttgtt tct
//
