LOCUS       sequence-50426-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: RAD Module G8-C1
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     266..283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(307..364)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    365..385
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg ccctagctag gattaccgcc tttgagtgag ctgataccgc
      241 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct gcataacgcg
      301 aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc ccttttttgc
      361 cggactgcag cggccgctac tagtattatt aatgacgagc agcgtagttt tcgtcgtttg
      421 ctgcgccggt cgacatcccg gtgtgtagcc gttcgaccac gctgccgagc ctgagatgct
      481 gctcgtactc ctgaagatcc ccgaagtcga tcgtgcgagt cagcccgccg cggacgtcga
      541 acgtcagccg aacgttcatc gaccgaagcc aggtgttctt tgccgcggtg tcctgctccc
      601 gccaccagtc cccgaaccgc tgcccggtct cgcgccactc ccagccagac gggcgagcct
      661 ccaggccctc cagctcctct tgccgcgcgg ccagcgccgc aatacgggca tccagtgctt
      721 ctcgctgcgg agagcccgcc cggtaggccg gggagccgat cagcgacgtc aggtccacca
      781 gctccgcgtt cacctccgcg agttcgaccg cggagtccga gcccgctacc cagactttct
      841 ccagacgctc c
//
