LOCUS       sequence-50427-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Beier KT, Samson ME, Matsuda T, Cepko CL
  TITLE     Conditional expression of the TVA receptor allows clonal analysis of
            descendents from Cre-expressing progenitor cells.
  JOURNAL   Dev Biol. 2011 May 15;353(2):309-20. Epub 2011 Mar 21.
  PUBMED    21397594
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2011-05-15"; volume: "353(2)"; pages: "309-20. Epub 2011 Mar 
            21"
COMMENT     Sequence Label: pQC mPlum IX
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             151..831
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     552..571
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     746..769
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctgttttgac ctccatagac agaaccggga ccgatccagc ctccgcggcc gggaacggtg
       61 cattggaacg gacctgcagg aattgatccg cggccgcacc ggtaggcctc gtacgcttaa
      121 ttaacggatc cccgggtacc ggtcgccacc atggtgagca agggcgagga ggtcatcaag
      181 gagttcatgc gcttcaagga gcacatggag ggctccgtga acggccacga gttcgagatc
      241 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaggct gaaggtgacc
      301 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagatcat gtacggctcc
      361 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      421 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      481 gactcctccc tgcaggacgg cgagttcatc tacaaggtga aggtgcgcgg caccaacttc
      541 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      601 atgtaccccg aggacggcgc cctgaagggc gagatgaaga tgaggctgag gctgaaggac
      661 ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg
      721 cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc
      781 atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccta gcggccgca
//
