LOCUS       sequence-50430-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Bonnet J, Subsoontorn P, Endy D
  TITLE     Rewritable digital data storage in live cells via engineered control
            of recombination directionality.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 
            21.
  PUBMED    22615351
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-06-5"; volume: "109(23)"; pages: 
            "8884-9. Epub 2012 May 21"
COMMENT     Sequence Label: pBad-Xis/Int Reset Flipper
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     266..283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(307..364)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    365..385
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg ccctagctag gattaccgcc tttgagtgag ctgataccgc
      241 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct gcataacgcg
      301 aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc ccttttttgc
      361 cggactgcag cggccgctac tagtagcgaa aaaaccccgc cgaagcgggg ttttttgcgc
      421 tagtattatt acgacatccc ggtgtgtagc cgttcgacca cgctgccgag cctgagatgc
      481 tgctcgtact cctgaagatc cccgaagtcg atcgtgcgag tcagcccgcc gcggacgtcg
      541 aacgtcagcc gaacgttcat cgaccgaagc caggtgttct ttgccgcggt gtcctgctcc
      601 cgccaccagt ccccgaaccg ctgcccggtc tcgcgccact cccagccaga cgggcgagcc
      661 tccaggccct ccagctcctc ttgccgcgcg gccagcgccg caatacgggc atccagtgct
      721 tctcgctgcg gagagcccgc ccggtaggcc
//
