LOCUS       sequence-50438-e        2189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_105_Venus_173
FEATURES             Location/Qualifiers
     source          1..2189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1386..2120
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     1523..1544
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1647..1668)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1908..1927)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggttcagga tccgaggaca agggcatcgt gaaggtgaag tccgacatct
      121 ccatggagga ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca
      181 acggccccgt gatgcagaag aagaccaccg gctgggacgc ctccaccgag aggatgtacg
      241 tgcgcgacgg cgtgctgaag ggcgacgtca agcacaagct gctgctggag ggcggcggcc
      301 accaccgcgt tgacttcaag accatctaca gggccaagaa ggcggtgaag ctgcccgact
      361 atcactttgt ggaccaccgc atcgagatcc tgaaccacga caaggactac aacaaggtga
      421 ccgtttacga gagcgccgtg gcccgcaact ccaccgacgg catggacgag ctgtacaagg
      481 gtgctagcgg aggaatggtg agcaagggcg aggagaccac aatgggcgta atcaagcccg
      541 acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc gtgatcgagg
      601 gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag gtgaaggagg
      661 gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac ggcaacaggg
      721 ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc cccgagggct
      781 actcttggga gcgcaccatg accttctccg gaatggcgga cgaggagaag ctgccgcccg
      841 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      901 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      961 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgcccg
     1021 ggtctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg
     1081 gcggtggtgg aggaggagga gctagaggcg gaggtggtgg aggaggtgga ggtggtggag
     1141 gtggaggtgg aggtggtggc ggtggtggag gaggaggagc cagcggcgga ggtggtggag
     1201 gaggtggagg tggtggaggt ggaggtggag gtggtggcgg tggtggagga ggaggaaccg
     1261 gtgctgacca actgactgaa gagtggtcga cggtacagcg gaccgcgcct gacgtaccac
     1321 gtacaccagt aggaaaggca aagctttcat tccaattccc gcgggcgcgg gacatcgcgg
     1381 ccgcgatgga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1441 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     1501 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1561 ctctcggcat ggacgagctg tacaagggtg gcagcggtgg catggtgagc aagggcgagg
     1621 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1681 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     1741 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     1801 acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1861 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1921 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1981 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2041 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     2101 agatccgcca caacatcgag ggtaccgata tccttcaaaa aaaacttgaa gaacttgaac
     2161 ttgacgaatg agatatctaa tgactcgag
//
