LOCUS       sequence-50442-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Jacinto E, Loewith R, Schmidt A, Lin S, Ruegg MA, Hall A, Hall MN
  TITLE     Mammalian TOR complex 2 controls the actin cytoskeleton and is 
            rapamycin insensitive.
  JOURNAL   Nat Cell Biol. 2004 Nov;6(11):1122-8. Epub 2004 Oct 3.
  PUBMED    15467718
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2004-11"; volume: "6(11)"; pages: "1122-8. Epub 2004 
            Oct 3"
COMMENT     Sequence Label: pCI-HA-mAVO3 (pRL62-3)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          145..277
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     260..279
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        322..340
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             358..384
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             364..390
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
       61 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga
      121 agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac
      181 caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt
      241 ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac
      301 agctcttaag gctagagtac ttaatacgac tcactatagg ctagcatggc ttctagctat
      361 ccttatgacg tgcctgacta tgccagcctg ggaggacctt ctgaattcac gcgcatggcg
      421 gcgatcagcc gcggccgctc tctgaagaac ctccgagtac gagggcggaa tgacagcggc
      481 gaggagaacg tcccgctcga tctgacccga gaaccttctg ataacttgag ggagattctc
      541 caaaatgtgg ccaaattgca aggagtatca aatatgagaa agctgggcca tctgaataac
      601 ttcactaagc ttctttgcga tattggccat agtgaagaaa aactgggctt taattatgaa
      661 gacatcataa tttgtttgcg cttagcttta ttaaatgagg caaaagaagt aagagcagcc
      721 gggttgcgag cgctgcgcta tctcatccaa gattccagta ttctccagaa ggtgctaaaa
      781 ctgaaagtgg actatttaat agctaggtgc attgacattc agcagagcaa cgaggtggag
      841 agga
//
