LOCUS       sequence-50445-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Wullschleger S, Loewith R, Oppliger W, Hall MN
  TITLE     Molecular organization of target of rapamycin complex 2.
  JOURNAL   J Biol Chem. 2005 Sep 2;280(35):30697-704. Epub 2005 Jul 7.
  PUBMED    16002396
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-09-2"; volume: "280(35)"; pages: "30697-704. 
            Epub 2005 Jul 7"
COMMENT     Sequence Label: pHAC111-LST8 (pSW75)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..199)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     211..229
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(361..387)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(388..414)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(418..444)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca
       61 tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa
      121 tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc
      181 cttttcggtt agagcggatg tggggggagg gcgtgaatgt aagcgtgaca taactaatta
      241 catgatatcg acaaaggaaa aggggcctgt ttactcacag gcttttttca agtaggtaat
      301 taagtcgttt ctgtcttttt ccttcttcaa cccaccaaag gccatcttgg tacgtaccta
      361 agcgtaatct ggaacgtcat atggatatgc atagtccggg acgtcatagg gatagcccgc
      421 atagtcagga acatcgtatg ggtagcatgc ctgcagtaca tcgtttaatg cgacacatac
      481 agctcccttg tggtgtccac cgtactgtct gacaatttct ctagttgata aatcccataa
      541 tctcacgtaa tgatcggacg atgccgttac tagatacgca ctgtcagcac taaatgcgca
      601 atcccaaacc cacctttggt ggccatccaa tgtcgtttct agcttaaaat catcatcaat
      661 cgaccacaca cgagcagtgt gatccgctga acatgttgcc aaatgtttta catcagatga
      721 tagtagaatt ctagttatat atgtggagtg cgctctgaat tttgttaccg gttttagatg
      781 ggatgcgtca gtatgatttg gcatttccca tacgtaacaa ttacctttag tatttgctg
//
