LOCUS       sequence-50449-e        2171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_175_Venus_wt
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1386..1388,1389..1391,1392..2102)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1431..1452)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1692..1711)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2039..2060
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggttcagga tccggcggcc accaccgcgt tgacttcaag accatctaca
      121 gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc
      181 tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact
      241 ccaccgacgg catggacgag ctgtacaagg gtgctagcgg aggaatggtg agcaagggcg
      301 aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca
      361 acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca
      421 ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc
      481 tgaccaccgc gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca
      541 actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg
      601 acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg
      661 agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca
      721 ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg
      781 tcaagcacaa gctgctgctg gagggctccg gaatggcgga cgaggagaag ctgccgcccg
      841 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      901 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      961 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgcccg
     1021 ggtctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg
     1081 gcggtggtgg aggaggagga gctagaggcg gaggtggtgg aggaggtgga ggtggtggag
     1141 gtggaggtgg aggtggtggc ggtggtggag gaggaggagc cagcggcgga ggtggtggag
     1201 gaggtggagg tggtggaggt ggaggtggag gtggtggcgg tggtggagga ggaggaaccg
     1261 gtgctgacca actgactgaa gagtggtcga cggtacagcg gaccgcgcct gacgtaccac
     1321 gtacaccagt aggaaaggca aagctttcat tccaattccc gcgggcgcgg gacatcgcgg
     1381 ccgcgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1441 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1501 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     1561 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     1621 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1681 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1741 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1801 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     1861 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     1921 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1981 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     2041 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     2101 agggtaccga tatccttcaa aaaaaacttg aagaacttga acttgacgaa tgagatatct
     2161 aatgactcga g
//
