LOCUS       sequence-50452-e        2189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_175_Venus_195
FEATURES             Location/Qualifiers
     source          1..2189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     1460..1481
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(1539..1541,1542..1544,1545..2057)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1584..1605)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1845..1864)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggttcagga tccggcggcc accaccgcgt tgacttcaag accatctaca
      121 gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc
      181 tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact
      241 ccaccgacgg catggacgag ctgtacaagg gtgctagcgg aggaatggtg agcaagggcg
      301 aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca
      361 acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca
      421 ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc
      481 tgaccaccgc gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca
      541 actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg
      601 acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg
      661 agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca
      721 ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg
      781 tcaagcacaa gctgctgctg gagggctccg gaatggcgga cgaggagaag ctgccgcccg
      841 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      901 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      961 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgcccg
     1021 ggtctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg
     1081 gcggtggtgg aggaggagga gctagaggcg gaggtggtgg aggaggtgga ggtggtggag
     1141 gtggaggtgg aggtggtggc ggtggtggag gaggaggagc cagcggcgga ggtggtggag
     1201 gaggtggagg tggtggaggt ggaggtggag gtggtggcgg tggtggagga ggaggaaccg
     1261 gtgctgacca actgactgaa gagtggtcga cggtacagcg gaccgcgcct gacgtaccac
     1321 gtacaccagt aggaaaggca aagctttcat tccaattccc gcgggcgcgg gacatcgcgg
     1381 ccgccatgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     1441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1501 tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag ggcgaggagc
     1561 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1621 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     1681 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     1741 gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1801 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1861 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1921 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1981 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     2041 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     2101 ccatcggcga cggccccgtg ggtaccgata tccttcaaaa aaaacttgaa gaacttgaac
     2161 ttgacgaatg agatatctaa tgactcgag
//
