LOCUS       sequence-50453-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Violin JD, Ren XR, Lefkowitz RJ
  TITLE     G-protein-coupled receptor kinase specificity for beta-arrestin 
            recruitment to the beta2-adrenergic receptor revealed by 
            fluorescence resonance energy transfer.
  JOURNAL   J Biol Chem. 2006 Jul 21;281(29):20577-88. Epub 2006 May 10.
  PUBMED    16687412
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-07-21"; volume: "281(29)"; pages: "20577-88. 
            Epub 2006 May 10"
COMMENT     Sequence Label: beta-₂AR-mCFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(6..719,720..722))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(51..72)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     400..419
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     659..680
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctagattact tgtacagctc gtccatgccg tgagtgatcc cggcggcggt cacgaactcc
       61 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc
      121 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      181 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gtgggccttg
      241 atgccgttct tctgcttgtc ggcggtgata tagacgttgt cgctgatggc gttgtactcc
      301 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      361 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      421 ttgaagaaga tggtacgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      481 tgctgcttca tgtggtcggg gtagcgggcg aagcactgca cgccccaggt cagggtggtc
      541 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
      601 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacctgtg gccgtttacg
      661 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
      721 accagtgcct cgagtccacc cagcagtgag tcatttgtac tacaattcct cccttgtgaa
      781 tcaatgttat cgctaggcac agtaccttga tggcccacaa
//
