LOCUS       sequence-50459-e        2189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_227_Venus_229
FEATURES             Location/Qualifiers
     source          1..2189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1434..1436,1437..1439,1440..1952)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1479..1500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1740..1759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2087..2108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggttcagga tcctccaccg acggcatgga cgagctgtac aagggtgcta
      121 gcggaggaat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga
      181 agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg
      241 gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc
      301 ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca
      361 ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt
      421 gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca
      481 tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc ccccccaacg
      541 gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg atgtacgtgc
      601 gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc
      661 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc
      721 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg
      781 tttacgagag cgccgtggcc cgcaactccg gaatggcgga cgaggagaag ctgccgcccg
      841 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      901 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      961 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgcccg
     1021 ggtctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg
     1081 gcggtggtgg aggaggagga gctagaggcg gaggtggtgg aggaggtgga ggtggtggag
     1141 gtggaggtgg aggtggtggc ggtggtggag gaggaggagc cagcggcgga ggtggtggag
     1201 gaggtggagg tggtggaggt ggaggtggag gtggtggcgg tggtggagga ggaggaaccg
     1261 gtgctgacca actgactgaa gagtggtcga cggtacagcg gaccgcgcct gacgtaccac
     1321 gtacaccagt aggaaaggca aagctttcat tccaattccc gcgggcgcgg gacatcgcgg
     1381 ccgccatgat cactctcggc atggacgagc tgtacaaggg tggcagcggt ggcatggtga
     1441 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1501 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1561 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1621 ccaccctggg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1681 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1741 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1801 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1861 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1921 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     1981 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2041 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2101 agttcgtgac cgccgccggg ggtaccgata tccttcaaaa aaaacttgaa gaacttgaac
     2161 ttgacgaatg agatatctaa tgactcgag
//
