LOCUS       sequence-50463-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ME1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg cagcgcggct acctgctgac
      121 acggaaccct cacctcaaca aggacttggc ctttaccctg gaagagagac agcaattgaa
      181 cattcatgga ttgttgccac cttccttcaa cagtcaggag atccaggttc ttagagtagt
      241 aaaaaatttc gagcatctga actctgactt tgacaggtat cttctcttaa tggatctcca
      301 agatagaaat gaaaaactct tttatagagt gctgacatct gacattgaga aattcatgcc
      361 tattgtttat actcccactg tgggtctggc ttgccaacaa tatagtttgg tgtttcggaa
      421 gccaagaggt ctctttatta ctatccacga tcgagggcat attgcttcag ttctcaatgc
      481 atggccagaa gatgtcatca aggccattgt ggtgactgat ggagagcgta ttcttggctt
      541 gggagacctt ggctgtaatg gaatgggcat ccctgtgggt aaattggctc tatatacagc
      601 ttgcggaggg atgaatcctc aagaatgtct gcctgtcatt ctggatgtgg gaaccgaaaa
      661 tgaggagtta cttaaagatc cactctacat tggactacgg cagagaagag taagaggttc
      721 tgaatatgat gattttttgg acgaattcat ggaggcagtt tcttccaagt atggcatgaa
      781 ttgccttatt cagtttgaag attttgccaa tgtgaatgc
//
