LOCUS       sequence-50474-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: OXSM
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gagattgcat aggcgagttg
      121 tcattacagg cattggctta gtgactcctc ttggtgttgg aactcacctg gtttgggatc
      181 gtcttatcgg aggagagagt ggaattgttt cactggttgg tgaagagtat aagagtatcc
      241 cttgcagtgt tgctgcttat gtgccaagag gtagtgatga aggtcagttc aatgaacaaa
      301 actttgtgtc caaatcagat atcaagtcca tgtcttctcc caccatcatg gccattgggg
      361 ctgcagaatt agccatgaag gattctggct ggcatcctca gtcagaagct gatcaagtgg
      421 ctactggtgt tgcaattggc atgggaatga ttcctcttga agttgtttct gaaactgctt
      481 tgaattttca gacaaaaggt tacaataaag ttagcccatt ttttgtccct aagattctgg
      541 tcaatatggc agcaggccag gtcagcattc gatataaact caagggccca aatcatgcag
      601 tatccacagc ctgtaccaca ggagctcatg ctgtgggaga ctcatttaga tttatagccc
      661 atggtgatgc tgatgtgatg gtggctggag gtacagattc ttgtattagc cctttatctc
      721 ttgctgggtt ttccagagcc cgggctctga gcacaaactc agatcccaag ttggcatgtc
      781 gaccatttca tccaaagaga gatggttttg taatgggaga aggtgcagct gtgctggtgc
      841 tggaagaata tgaa
//
