LOCUS       sequence-50490-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX1
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(676..696)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(721..738)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             749..771
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    786..810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(810..829)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(811..829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatactcttt gggctttgtt gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatccgt atccaccttt actgtcattg
      121 agacagcagg gcttcgatgt caggctcgat gtcaatggtc tggaagcggc ggctgtacct
      181 gcgtaggggc acaccgtcag ggcccaccag gaacttctca aagttccagg caacatcgtt
      241 gcgacacacc ggagaccagg tgatgagctt ggggtcggtc ataagcgcgg tggcgtcgtc
      301 gctgggagct ggcagggcct cccgcaggaa ggcgaagaga gggtgcgccc ccgcaccgtt
      361 cacctcgcac ttctcgaaga gcatgaagtt gggctcgaac ccaccaccag gccggacgta
      421 cttgagggaa ttcagaatct cttcgttctt ggcgttctcc tgatgcccaa actggttgca
      481 cgggaagccg agcaccacca ggccccgggg tccgaggcgc cgctgcagct cgttcatctg
      541 ggtgtagtcc cggaccgtgg tgcctccgag ggacgccaca ttctcgataa gtagtacctt
      601 gccccgcagg gagcccaggc tcacaggctc cccgccggcc agcgggcgcg ccgagaaggc
      661 atacaccgac tgcatggatt ggaagtacag gttctcggta cccagatcta caccagaaga
      721 atgatgatga tgatggtgca tatgtatatc tccttcttaa agttaaacaa aattatttct
      781 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga
      841 tcgaga
//
