LOCUS       sequence-50491-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX1
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             720..737
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(790..808)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      804..851
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt
       61 gtagatctgg gtaccgagaa cctgtacttc caatccatgc agtcggtgta tgccttctcg
      121 gcgcgcccgc tggccggcgg ggagcctgtg agcctgggct ccctgcgggg caaggtacta
      181 cttatcgaga atgtggcgtc cctcggaggc accacggtcc gggactacac ccagatgaac
      241 gagctgcagc ggcgcctcgg accccggggc ctggtggtgc tcggcttccc gtgcaaccag
      301 tttgggcatc aggagaacgc caagaacgaa gagattctga attccctcaa gtacgtccgg
      361 cctggtggtg ggttcgagcc caacttcatg ctcttcgaga agtgcgaggt gaacggtgcg
      421 ggggcgcacc ctctcttcgc cttcctgcgg gaggccctgc cagctcccag cgacgacgcc
      481 accgcgctta tgaccgaccc caagctcatc acctggtctc cggtgtgtcg caacgatgtt
      541 gcctggaact ttgagaagtt cctggtgggc cctgacggtg tgcccctacg caggtacagc
      601 cgccgcttcc agaccattga catcgagcct gacatcgaag ccctgctgtc tcaatgacag
      661 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      721 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      781 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      841 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggaac gcgcccctgg
      901 ta
//
