LOCUS       sequence-50501-e         563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression vector with Gateway cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCXLE-gw
FEATURES             Location/Qualifiers
     source          1..563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(43..48,49..66,67..73)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(193..212)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
       61 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      121 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      181 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      241 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggtc
      301 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      361 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      421 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      481 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      541 attgagaata tgtttttcgt ctc
//
