LOCUS       sequence-50505-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(445..465)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(490..507)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             518..540
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    555..579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(579..598)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(580..598)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(682..701)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctccttcggg cttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcactgaaa
      121 ttctttcctc ttactttttt ttaaataaca ctgatgaact agtgggagca attttccttc
      181 tttgtgaagc ctatgagtgt cagttgcacc tccaataaaa gtaccattga caaatattct
      241 tggaacagtt ctttcaccag tcattttgta aagagcatct tggaactggt ttccatattc
      301 aagcaggtcc agttccacca ctttatagtt aacattcatg tcatggaaaa gcttttttgc
      361 cattgtacag taagaacagg atgtttttga gaaaatcacc acacaattat cagaaattgt
      421 ttcttggatc tggttcacag gcatggattg gaagtacagg ttctcggtac ccagatctac
      481 accagaagaa tgatgatgat gatggtgcat atgtatatct ccttcttaaa gttaaacaaa
      541 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
      601 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
      661 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      721 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
      781 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
      841 gcctcaacct actactggcg ctctatgaga tccaaagaac gca
//
