LOCUS       sequence-50513-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HIBCH
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(97..120)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(121..138)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(139..159)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctcttcgggc ttgttgcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tcctcactta tcgtcgtcat ccttgtaatc
      121 atggtggtga tgatggtgcg attggaagta gaggttctct gcaaatttca aatcactgct
      181 tcccaaagac ttaaagtgat tattcaaatc ttcctcagta acttctttta gatcagctgg
      241 tttccatttt ggactctggt ctttatcaat taaaacagct ctaacgcctt catgaaagtc
      301 atgacctctc atacaagctt gacttagccg atactccata gttagtactt cttgcaaggt
      361 ctttgaagac ccctccatga gttgccttag tgtgatcttt agagatgttg gagacatttt
      421 attaattacc ttcaattgct ctagggcaaa agatgaacca tcttgctgta agttttcaat
      481 aatttcttcc acagtattgg ctgaaaaaca actgtttatt ttgtccatgt gttcctcaag
      541 tataaaagac ttgtctcgat caatcttaga ctctgtatgg taattttcta agacagatgc
      601 aatattttct tttgaaggag atttcaaggc taacaaatct tcctctaaca tggccaactt
      661 ttcagaatct acaaagtgtg tagcaattcc tgctctgtac acatctcttc cttttagtct
      721 gaatcctgtt aatgcaagga agtaaccaag ttttccttgg agtcgtggca agaaataacc
      781 tccacccaca tcagggaaca gtcctattgc agtttctggc ata
//
