LOCUS       sequence-50518-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SOCS6
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..57)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(543..563)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(588..605)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             616..638
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    653..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(677..696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(678..696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcatacttcc tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc
       61 gagtgcggcc gcaatcttgt cgacggagct cgaattcgga tccgtatcca cctttactgt
      121 cacgagcgca cctgcatgaa ccgggacact gggttggtca gtctgacggg gtaagttgca
      181 gatccaggca gccgagacct tgaataacaa aaagctccat tttcagagtc cccgattgaa
      241 tgctcaatta gatcaactat ggacgtatgt ccttccacat ctggctgttc ataaaagcta
      301 aacctaccat ttgagtgctc aattctagtg tgaagtgttt taccatggga gcgaaagctc
      361 aagcttaaaa ggtaacggtc gtcagaactg tcccgaacaa gaaaagaacc atctggcacg
      421 tttgctagct tcccttctgc ctcccaacgt gtgattggtc cccagtacca tccttgcttt
      481 gcaagttttt tcagctcctc tgtaaggctt gtcacatcca tgggaccact actttgcacc
      541 atggattgga agtacaggtt ctcggtaccc agatctacac cagaagaatg atgatgatga
      601 tggtgcatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      661 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat
      721 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      781 ctatatcgcc cgacatcacc
//
