LOCUS       sequence-50519-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SOCS6
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             600..617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(670..688)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      684..731
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgggaattcc tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggtg
      121 caaagtagtg gtcccatgga tgtgacaagc cttacagagg agctgaaaaa acttgcaaag
      181 caaggatggt actggggacc aatcacacgt tgggaggcag aagggaagct agcaaacgtg
      241 ccagatggtt cttttcttgt tcgggacagt tctgacgacc gttacctttt aagcttgagc
      301 tttcgctccc atggtaaaac acttcacact agaattgagc actcaaatgg taggtttagc
      361 ttttatgaac agccagatgt ggaaggacat acgtccatag ttgatctaat tgagcattca
      421 atcggggact ctgaaaatgg agctttttgt tattcaaggt ctcggctgcc tggatctgca
      481 acttaccccg tcagactgac caacccagtg tcccggttca tgcaggtgcg ctcgtgacag
      541 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      601 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      661 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      721 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      841 cgccctagcg cccgctcctt tcgctttctt ccccttcc
//
