LOCUS       sequence-50523-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, 
            Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van 
            Oudenaarden A, Weinberg RA
  TITLE     Slug and Sox9 cooperatively determine the mammary stem cell state.
  JOURNAL   Cell. 2012 Mar 2;148(5):1015-28.
  PUBMED    22385965
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-03-2"; volume: "148(5)"; pages: "1015-28"
COMMENT     Sequence Label: FUW-Sox10
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(298..322)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(430..454)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(451..489)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(522..540,541..563,564..582,583..605,606..624,625..647,
                     648..666,667..689,690..708,709..731,732..750,751..773,
                     774..792)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 tgctggcgcc gttgacgcgc acgggcatgg gcaccagcgt ccagtcgtag ccgctgagca
       61 cctggctgac ggcctcgcgg atgcacacgg ggaacttgtc atcgtccgcc tcgccgtcct
      121 gctgctcctt cttgaccttg cccagctcgc ctggccccgg gctggctcgc aggcccgatc
      181 cgccgccgcc gccgtcgggc cctagcgagg gcgcgctccc cggggacagg cagcggggct
      241 cctccgagcc cacggggctc agctccacct ccgatagatc ctgctcctcc gccatggtgc
      301 caactttttt gtacaaactt gttgatatct gcagaattcc accacactgg gcaattcggg
      361 gccgcggagg ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg
      421 tctccaggcg atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac
      481 acgcctacct cgacccgggt accgagctcg actttcactt ttctctatca ctgataggga
      541 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
      601 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
      661 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
      721 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      781 cactgatagg gagtggtaaa ctcgagatct aaccaaactg gatctctgct gtccctgtaa
      841 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
      901 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc ccc
//
