LOCUS       sequence-50545-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, 
            Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van 
            Oudenaarden A, Weinberg RA
  TITLE     Slug and Sox9 cooperatively determine the mammary stem cell state.
  JOURNAL   Cell. 2012 Mar 2;148(5):1015-28.
  PUBMED    22385965
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-03-2"; volume: "148(5)"; pages: "1015-28"
COMMENT     Sequence Label: pLV-beta-catenin deltaN90
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..124)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    242..359
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          416..619
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(439..459)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gggactgcat gccctcatct aatgtctcag ggaacatagc agctcgtacc ctctgagctc
       61 gaatctgcag aattcgtcga catcgatgga tcctcaggtc ctcctcggag atcagcttct
      121 gctccatggg attgacgcgt gtcttctatg gaggtcaaaa cagcgtggat tcaactatct
      181 atcgcgtaag cttaggcctg gatcccgtag tttaaacctc gaggctagtc tcgtgatcga
      241 taaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
      301 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag
      361 ttaaccgata ccgtcgagat taattaaatt taaatgtcga aattcctcac gacacctgaa
      421 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
      481 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
      541 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
      601 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
      661 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
      721 ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa
      781 gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc cgcccagggc
      841 ggggcgatac acggcgggcg cgaggccagg caccagagca ggccgggcca gcttgagact
      901 acccccgtcc gattctcgg
//
