LOCUS       sequence-50551-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Sano T, Pandori MW, Chen X, Smith CL, Cantor CR
  TITLE     Recombinant core streptavidins. A minimum-sized core streptavidin 
            has enhanced structural stability and higher accessibility to 
            biotinylated macromolecules.
  JOURNAL   J Biol Chem. 1995 Nov 24. 270(47):28204-9.
  PUBMED    7499314
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Nov 24. 270(47):28204-9."
COMMENT     Sequence Label: pTSA-13
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             531..563
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(617..635)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      631..678
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatataca tatgggcatc accggcacct ggtacaacca gctcggctcg accttcatcg
      181 tgaccgcggg cgccgacggc gccctgaccg gaacctacga gtcggccgtc ggcaacgccg
      241 agagccgcta cgtcctgacc ggtcgttacg acagcgcccc ggccaccgac ggcagcggca
      301 ccgccctcgg ttggacggtg gcctggaaga ataactaccg caacgcccac tccgcgacca
      361 cgtggagcgg ccagtacgtc ggcggcgccg aggcgaggat caacacccag tggctgctga
      421 cctccggcac caccgaggcc aacgcctgga agtccacgct ggtcggccac gacaccttca
      481 ccaaggtgta gccgtccgcc gcctccatcg acgcggcgaa gaaggcccat atggctagca
      541 tgactggtgg acagcaaatg ggtcgcggat ccggctgcta acaaagcccg aaaggaagct
      601 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      661 gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt
      721 ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
      781 gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatca
//
