LOCUS       sequence-50552-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Nakamura T, Liu YJ, Nakashima H, Umehara H, Inoue K, Matoba S, 
            Tachibana M, Ogura A, Shinkai Y, Nakano T
  TITLE     PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 
            5hmC in early embryos.
  JOURNAL   Nature. 2012 Jun 3;486(7403):415-9. doi: 10.1038/nature11093.
  PUBMED    22722204
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-06-3- 3"; volume: "486"; issue: "7403"; pages:
            "415-9"
COMMENT     Sequence Label: pcDNA4-FLAG-Jhdm2a
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..106
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gttaagctta tggactacaa agacgatgac gacaagggat ccatgtacaa
      121 atctcttcta gacaaagctg gcttgggagc cataacttct gttcggtttc ttggagatca
      181 acaaagtgta tttgtttcca aagacctttt gaaacctata caggatgtta acagtcttcg
      241 gctttccctt actgataatc agacagtcag taaggaattt caagctttga ttgtaaaaca
      301 tttggatgaa agccatcttt tacaaggtga caagaacctt gttggttcag aagtaaaaat
      361 ttatagcttg gacccatcta ctcagtggtt ttcagcaact gttgtacatg gaaacccatc
      421 atccaaaact cttcaagtca actgtgagga gattccagca ctgaaaattg tcgacccagc
      481 actgattcat gttgaagttg tacatgacaa ctttgtgaca tgtggtaatt ctacaagaac
      541 tggagctgta aaacgcaagt cttctgagaa taacggaagt tcggtttcta aacaagcaaa
      601 atcttgttct gaggcctctc ccagtatgtg tcctgtacag tctgttccca caacagtgtt
      661 taaggagatc ctgcttggct gtactgcagc aactccatct agcaaggacc caagacagca
      721 aaatactccc caggcagcca attctccacc taacattgga gcaaaacttc ctcaaggatg
      781 tc
//
