LOCUS       sequence-50557-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DVL2
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             360..377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(430..448)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      444..491
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(616..635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgctcaata tcatcacagt cacgctaaac atggagaagt acaacttcct gggtatctcc
       61 attgttggcc agagcaatga gcggggagac ggaggcatct acattggctc catcatgaag
      121 ggtggggctg tggcggccga cgggcgcatt gagccagggg acatgctttt gcaggtgaat
      181 gacatgaact ttgagaacat gagcaacgat gacgctgtgc gggtgctgag ggacattgtg
      241 cacaagcctg gccccattgt gctgactgtg gccaagtgct gggaaacttc tgtttgacag
      301 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      361 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      421 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      481 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      601 cgccctagcg ccccgctcct ttcgctttct tccctttcct ttctcgccac gtttcgccgg
      661 ctttccccgt caagctgtaa atcggggggg ctcccctttt a
//
