LOCUS       sequence-50560-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM4
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(379..399)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(424..441)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             452..474
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    489..513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(513..532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(514..532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(616..635)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctcttcggct tgtagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 cagtcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc acaagtcaga
      121 ttcgccctca ggcctgctca cagacagtgt gaggtgatcg tggcagccct tgatgcggtt
      181 ctgtgcctcc aggtgtgtca tgagctctgt gctctcacca ttgatggcct ggatcaggtc
      241 tcctgggcac agggcagcca atgcagcctt gctgccagca tggacccgtg agatggtgag
      301 gggcgcgctg aagtcccggc cgcccaccag gcggaagccc cagggcgaag gcccgcgcag
      361 ggtcacggaa tggggcatgg attggaagta caggttctcg gtacccagat ctacaccaga
      421 agaatgatga tgatgatggt gcatatgtat atctccttct taaagttaaa caaaattatt
      481 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      541 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      601 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      661 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      721 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
      781 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg
      841 gacaccatcg
//
