LOCUS       sequence-50561-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM4
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             438..455
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(508..526)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      522..569
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(693..712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     904..925
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat
       61 tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgcc ccattccgtg
      121 accctgcgcg ggccttcgcc ctggggcttc cgcctggtgg gcggccggga cttcagcgcg
      181 cccctcacca tctcacgggt ccatgctggc agcaaggctg cattggctgc cctgtgccca
      241 ggagacctga tccaggccat caatggtgag agcacagagc tcatgacaca cctggaggca
      301 cagaaccgca tcaagggctg ccacgatcac ctcacactgt ctgtgagcag gcctgagggc
      361 gaatctgact tgtgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc
      421 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      481 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      541 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      601 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      661 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      721 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      781 attttgtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg ggttcacgta
      841 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      901 atagtggact cttgttccaa actggaacaa cactcaa
//
