LOCUS       sequence-50565-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM7
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(379..399)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(424..441)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             452..474
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    489..513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(513..532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(514..532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(616..635)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctctttgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcacaaaga
      121 agtaatggcc ctgctgaggc ccaggctgag gcgctccccg caggcccgga tcttgttctg
      181 agcttcgatg tgtgtgaggc tacccgcatt ctcgccatcg atgctcagca cccagtcacc
      241 cacggccact ccggcctgcg ccgctttgcc cccaggagtg agccgggaaa tggagagggg
      301 cacattgaag tccttgcccc cttgcagccg gaagccccaa ggtgctggcc cctccagcac
      361 tactttgaag gaatccatgg attggaagta caggttctcg gtacccagat ctacaccaga
      421 agaatgatga tgatgatggt gcatatgtat atctccttct taaagttaaa caaaattatt
      481 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      541 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      601 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      661 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      721 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
      781 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg
      841 gacaccatcg aatggcgcaa aactttcgcg tatgcagaac gccgaaaata tcgggaaaaa
      901 a
//
