LOCUS       sequence-50570-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Nabhan JF, Pan H, Lu Q
  TITLE     Arrestin domain-containing protein 3 recruits the NEDD4 E3 ligase to
            mediate ubiquitination of the beta2-adrenergic receptor.
  JOURNAL   EMBO Rep. 2010 Aug;11(8):605-11. Epub 2010 Jun 18.
  PUBMED    20559325
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2010-08"; volume: "11(8)"; pages: "605-11. Epub 2010 Jun 18"
COMMENT     Sequence Label: pEF6.HA-ARRDC3
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttggta
       61 ccgagctcgg atccaccgcc atgtacccat acgacgtccc agactacgct gtgctgggaa
      121 aggtgaagag tttgacaata agctttgact gtcttaatga cagcaatgtc cctgtgtatt
      181 ctagtgggga taccgtctca ggaagggtaa atttagaagt tactggggaa atcagagtaa
      241 aatctcttaa aattcatgca agaggacatg cgaaagtacg ctggactgaa tctagaaacg
      301 ccggctccaa tactgcctat acacagaatt acactgaaga agtagagtat ttcaaccata
      361 aagacatctt aattgggcac gaaagagatg atgataattc cgaagaaggc ttccacacta
      421 ttcattcagg aaggcatgaa tatgcattca gcttcgagct tccacagaca ccactcgcta
      481 cctcattcga aggccgacat ggcagtgtgc gctattgggt gaaagccgaa ttgcacaggc
      541 cttggctact accagtaaaa ttaaagaagg aatttacagt ctttgagcat atagatatca
      601 acactccttc attactgtca ccccaagcag gcacaaaaga aaagacactc tgttgctggt
      661 tctgtacctc aggcccaata tccttaagtg ccaaaattga aaggaagggc tataccccag
      721 gtgaatcaat tcagatattt gctgagattg agaactgctc ttcccgaatg gtggtgccaa
      781 aggcagccat ttaccaaaca caggccttct atgccaaagg gaaaatgaag gaagtaaaac
      841 agcttgtggc taacttgcgt ggggaatcct tatcatctgg aaagacagag acgtggaatg
      901 gcaagttgct gaaaattcca ccagtttctc cctctatcct cgactgtag
//
