LOCUS       sequence-50592-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-21a(+)/MBP-His
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(678..689)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgaaaat cgaagaaggc aaactggtca
       61 tctggatcaa tggcgacaaa ggctacaatg gtctggcgga agtgggcaaa aaattcgaaa
      121 aagataccgg cattaaagtg acggttgaac atccggacaa actggaagaa aaatttccgc
      181 aggtggcagc aaccggtgat ggtccggaca ttatcttttg ggcgcacgat cgtttcggcg
      241 gttatgcaca gtctggcctg ctggctgaaa tcaccccgga taaagccttt caagacaaac
      301 tgtacccgtt cacgtgggat gcagtgcgct ataatggtaa actgattgcg tacccgatcg
      361 ccgttgaagc actgtccctg atttataaca aagatctgct gccgaatccg ccgaaaacct
      421 gggaagaaat cccggcgctg gacaaagaac tgaaagcgaa gggtaaatca gccctgatgt
      481 ttaacctgca ggaaccgtat ttcacgtggc cgctgattgc agctgatggc ggttacgcgt
      541 tcaaatacga aaacggcaaa tacgatatca aagacgtcgg tgtggataac gcgggcgcca
      601 aagcaggtct gacctttctg gtcgacctga ttaaaaacaa acacatgaat gcggatacgg
      661 actattcgat cgccgaagcg gccttcaata aaggcgaaac cgcaatgacg attaacggtc
      721 cgtgggcttg gtccaatatc gatacctcaa aagttaacta cggcgttacc gtcctgccga
      781 cgtttaaagg tcaaccgagc aaaccgttcg tgggcgttct
//
