LOCUS       sequence-50595-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDZD3
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(402..422)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(447..464)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             475..497
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    512..536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(536..555)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(537..555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(639..658)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 acttctttgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg agctcgaatt cggatccgta tccaccttta ctgtcaaacg
      121 ctggtttcgc ggtccgcctc agggtcgacg acagtgaggg agacacagga gccctgcccc
      181 tggatcctgg acactgtctc ctcatggccc agcccctcca cgctctcccc agccacagcc
      241 accagccggt ccccagcctg catcccagcc ttcttggctg gcagtcccgg gtccacctcc
      301 cacaggaact gtccagggcg accgtcaagg cccttttcct cccggagcag gaacccaaaa
      361 ccctggggcc ctttctccag gtgcaggcag cggggcttca tggattggaa gtacaggttc
      421 tcggtaccca gatctacacc agaagaatga tgatgatgat ggtgcatatg tatatctcct
      481 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta
      541 tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt
      601 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
      661 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
      721 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
      781 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
      841 taagggagag cgtcgatatc ccggacacca tcgaatgg
//
