LOCUS       sequence-50597-e         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SYNJ2BP
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(331..351)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(376..393)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             404..426
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    441..465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(465..484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(466..484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattcggatc cggatccacc tttactgtca gatactgctt tctaacctgt gctgcactct
       61 cagagacaca gcatagcctg cattacgaaa gaggtctaca gcatcctggt gcagcaggtt
      121 ctttaggtct tggccattta ccgaaaggat cttatcaccc tcctggagcc gcccatccag
      181 ggccgcagcc ccattttctt tgatgcggct gacgtagatg ccactgtcgt tggagacata
      241 ctgctgatct gtcccaccga cgatgttgaa gcccagccct gagggccctc tggtaagatt
      301 gatctcttcc tcagtgacca aataatccat ggattggaag tacaggttct cggtacccag
      361 atctacacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt cttaaagtta
      421 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      481 attaatttcg cgggatcga
//
