LOCUS       sequence-50598-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFastBac1/MBP-His
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(187..204)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      387..396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
       61 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagta
      121 cttctcgaca agcttggtac cgcatgcctc gagactgcag gctctagatt cgaaagcggc
      181 cgcttagtgg tggtggtggt ggtgacccaa gttgttgttg ttgttgttgt tgttgttgtt
      241 gctagaggag ttagtttggg catccttcag agcctcgtcg acggtctgac gaccggaggc
      301 agcgttgatc acggcagtac gaacagcgta ccagaaggca gacatttgag ggatgttggg
      361 catgatctcg cccttctggg cgttttccat ggtggcagcg atgcgcgggt ccttcaccag
      421 ctcttcctcg taagacttca gagcaacggc tcccaatggc ttatccttgt tgacagcctc
      481 caagccttcg tcagtcaaga ggtagttctc gaggaattcc ttggccagtt ccttgttggg
      541 ggaggcagcg ttgataccag ccgagaggac tcccacgaac ggcttggatg gttgaccctt
      601 gaaggttggc agaactgtga ctccgtagtt gacctttgag gtgtcgatgt tgctccaggc
      661 ccaagggccg ttgatagtca tagcggtctc gcccttgttg aaggcagctt cggcgatgct
      721 gtaatctgtg tcagcgttca tgtgcttgtt cttgatgaga tcaaccagga aagtcaagcc
      781 agccttggca ccagcgttgt ccactccaac atccttgatg tcgtacttgc cgttct
//
