LOCUS       sequence-50602-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAGI1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(372..392)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(417..434)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             445..467
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    482..506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(506..525)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(507..525)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(609..628)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacggagctc gaattcggat ccgtatccac ctttactgtc aaacgctggt ttcaccacgt
      121 ttcagaaaca gacggacgcg acggccgcca tttttgatca gttcaatggc gcgagagtgc
      181 ttcatatttt tcgtggtctc gccgttgatt tccagaattt catcgccaat acgcatttta
      241 cccgagcgct cggccggacc gtcttcggcc agacgcagga cgtacaggtc catattatat
      301 tcacgtccac cacgcaggct aaaaccgaaa cctttcgcgc cacgttccag ttccaccgta
      361 taaaagtcca tggattggaa gtacaggttc tcggtaccca gatctacacc agaagaatga
      421 tgatgatgat ggtgcatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      481 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga
      541 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      601 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      661 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      721 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      781 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca
      841 tcgaatggcg caaaaccttt
//
