LOCUS       sequence-50603-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GRIP1
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(394..414)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(439..456)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             467..489
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    504..528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(528..547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(529..547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(631..650)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcaaaccg cggtttcact
      121 cacatcgtac tcaatcagca gagcagcttc ctgaccacac tgcttcagaa tactcatggc
      181 ttccgcatgc gtggtaccca gcaggcggat accatcaacg gacagcaggc gatcacccgg
      241 tttaatggtg ccttcacgat ccgccggacc gcccgggcgc acgctggtaa tcaccaccgg
      301 acggctttta ttgcgatcat catgcgcgcc accacgaatc acaaagccaa aggtattgcc
      361 ttctttatgc agggtcactt ccacggtgcg catggattgg aagtacaggt tctcggtacc
      421 cagatctaca ccagaagaat gatgatgatg atggtgcata tgtatatctc cttcttaaag
      481 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      541 cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca
      601 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
      661 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
      721 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
      781 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
      841 agcgtcgaga tcccgggaca catcgatgcg aaacttc
//
