LOCUS       sequence-50605-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Qin Y, Dittmer PJ, Park JG, Jansen KB, Palmer AE
  TITLE     Measuring steady-state and dynamic endoplasmic reticulum and Golgi 
            Zn2+ with genetically encoded sensors.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Epub 2011 Apr 
            18.
  PUBMED    21502528
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-05-3"; volume: "108(18)"; pages: 
            "7351-6. Epub 2011 Apr 18"
COMMENT     Sequence Label: pcDNA-Golgi-ZapCY1
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(3..5,6..518)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     HFKIRHNIE"
     primer_bind     complement(45..66)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(306..325)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     653..674
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
       61 gcgacgtaaa cggccacagg ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      121 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      181 tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      241 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgt accatcttct
      301 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      361 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      421 agctggagta caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg
      481 gcatcaaggc ccacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      541 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      601 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      661 tgctggagtt cgtgaccgcc gcccgcatgc ataaaaacaa tgacttaaaa tgcaaatgga
      721 aagaatgtcc tga
//
