LOCUS       sequence-50607-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  TITLE     Foxd3-pIRES2 eGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Foxd3-pIRES2 eGFP
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    34..496
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(194..213)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     423..440
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tcctcgccct tgctcaccat ggttgtggcc atattatcat cgtgtttttc aaaggaaaac
       61 cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa
      121 gcatgtgtac cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt
      181 cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact
      241 cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg
      301 gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga
      361 cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc
      421 ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc
      481 aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac
      541 aaacgcacac cggccttatt ccaagcggct tcggc
//
