LOCUS       sequence-50623-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre activity reporter plasmid; Zsgreen is expressed in cells without
            Cre, mCherry is expressed with Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Yang YS, Hughes TE
  TITLE     Cre stoplight: a red/green fluorescent reporter of Cre recombinase 
            expression in living cells.
  JOURNAL   Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.
  PUBMED    11730010
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2001-11"; volume: "31(5)"; pages: "1036, 
            1038, 1040-1"
COMMENT     Sequence Label: Cre Stoplight 2.4
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(407..426)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     676..694
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
       61 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcgggtgga atcgaattcc
      121 taggcgcgcc ccttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
      181 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
      241 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
      301 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
      361 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
      421 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
      481 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      541 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
      601 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
      661 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
      721 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
      781 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cct
//
