LOCUS       sequence-50624-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a 2A peptide construct that produces two different proteins 
            from a single open reading frame, a nuclear targeted Cre 
            recombinase, and a membrane targeted GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  TITLE     Cre Shine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Cre Shine
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(38..59)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(194..706,707..709,710..712))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKXXHNIE"
     primer_bind     387..406
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     646..667
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caactcgatc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
       61 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
      121 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      181 gcacgctgcc gtcctcgatg ttgtggcnna tcttgaagtt caccttgatg ccgttcttct
      241 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
      301 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
      361 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
      421 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      481 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
      541 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
      601 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
      661 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atctcgagaa
      721 tcttctggtc tgcatcctca tttt
//
