LOCUS       sequence-50634-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, 
            Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, 
            Hannon GJ, Lowe SW
  TITLE     A rapid and scalable system for studying gene function in mice using
            conditional RNA interference.
  JOURNAL   Cell. 2011 Apr 1;145(1):145-58.
  PUBMED    21458673
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-04-1"; volume: "145(1)"; pages: "145-58"
COMMENT     Sequence Label: pTGMP-p16/p19.478
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           47..168
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           196..210
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           238..364
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        378..877
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(413..436)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     810..829
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 agctgtcagt aatgaaccgg tcgactaggg ataacagggt aattgtttga atgaggcttc
       61 agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca
      121 ggttaaccca acagaaggct cgagaaggta tattgctgtt gacagtgagc gcccgctggg
      181 tgctctttgt gtttagtgaa gccacagatg taaacacaaa gagcacccag cggatgccta
      241 ctgcctcgga attcaagggg ctactttagg agcaattatc ttgtttacta aaactgaata
      301 ccttgctatc tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca
      361 cttttttcaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
      421 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
      481 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
      541 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
      601 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
      661 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
      721 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
      781 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
      841 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcc
//
