LOCUS       sequence-50635-e        1682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Mansfeld J, Collin P, Collins MO, Choudhary JS, Pines J
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly 
            checkpoint responsive to kinetochore attachment.
  JOURNAL   Nat Cell Biol. 2011 Sep 18;13(10):1234-43. doi: 10.1038/ncb2347.
  PUBMED    21926987
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-09-18- 18"; volume: "13"; issue: "10"; 
            pages: "1234-43"
COMMENT     Sequence Label: pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(401..975,976..978,979..987)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(568..585)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     795..814
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             988..1671
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MANSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIK
                     VIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVIT
                     VMQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMAL
                     KVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKF
                     AGLGGG"
ORIGIN
        1 ctcgagctca agcttcgaat tctgaccatg tccactttgt tcccctcact cttccctcgt
       61 gtgactgaga ctctgtggtt taatctggat cgaccctgtg tggaagagac agagctgcag
      121 cagcaggaac agcagcatca ggcctggctc caaagcatcg cggagaaaga caacaacctg
      181 gttcctattg gcaagccagc ctcagagcac tatgatgacg aggaagaaga ggatgatgaa
      241 gatgatgagg atagtgaaga ggactcagag gatgatgagg atatgcagga catggacgag
      301 atgaatgact acaatgagtc accggatgat ggagaggtca atgaggtgga catggaaggc
      361 aacgaacagg atcaggacca gtggatgatc tagggatccg cccctctccc tccccccccc
      421 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
      481 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
      541 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
      601 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
      661 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
      721 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      781 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      841 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
      901 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      961 aaacacgatg ataatatggc cacaaccatg gcaaacagcc tgatcaaaga aaacatgcgg
     1021 atgaaggtgg tgctggaagg cagcgtgaac ggccaccagt tcaagtgcac cggcgagggc
     1081 gagggcaacc cctacatggg cacccagacc atgcggatca aagtgatcga gggcggacct
     1141 ctgcccttcg ccttcgacat cctggccaca tccttcatgt acggcagccg gaccttcatc
     1201 aagtacccca agggcatccc cgatttcttc aagcagagct tccccgaggg cttcacctgg
     1261 gagagagtga ccagatacga ggacggcggc gtgatcaccg tgatgcagga caccagcctg
     1321 gaagatggct gcctggtgta ccatgcccag gtcaggggcg tgaattttcc cagcaacggc
     1381 gccgtgatgc agaagaaaac caagggctgg gagcccaaca ccgagatgat gtaccccgct
     1441 gacggcggac tgagaggcta cacccacatg gccctgaagg tggacggcgg agggcacctg
     1501 agctgcagct tcgtgaccac ctaccgatcc aagaaaaccg tgggcaacat caagatgccc
     1561 ggcatccacg ccgtggacca ccggctggaa aggctggaag agtccgacaa cgagatgttc
     1621 gtggtgcagc gggagcacgc cgtggccaag ttcgccggcc tgggcggagg gtaagcggcc
     1681 gc
//
