LOCUS       sequence-50637-e        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pYD1-mSA
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             7..16
                     /estimated_length=10
     CDS             80..421
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             443..466
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(796..815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(797..815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(822..839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(822..838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(831..853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 anntncnnnn nnnnnnctgc aggctagtgg tggtggtggt tctggtggtg gtggttctgg
       61 tggtggtggt tctgctagcg cggaagcggg tatcaccggc acgtggtaca accagtctgg
      121 ttctaccttc accgttaccg cgggtgcgga cggtaacctg accggtcagt acgaaaaccg
      181 tgcgcagggc actggttgcc agaactctcc gtacaccctg accggtcgtt acaacggtac
      241 caaactggaa tggcgtgttg aatggaacaa ctctaccgaa aactgccact ctcgtaccga
      301 atggcgtggt cagtaccagg gtggtgcgga agcgcgtatc aacacccagt ggaacctgac
      361 ctacgaaggt ggttctggtc cggcgaccga acagggtcag gacaccttca ccaaagttaa
      421 accgtctgcg gcgtctggat ccgactacaa ggacgatgac gacaagtaac tcgagatctg
      481 ataacaacag tgtagatgta acaaaatcga ctttgttccc actgtacttt tagctcgtac
      541 aaaatacaat atacttttca tttctccgta aacaacatgt tttcccatgt aatatccttt
      601 tctatttttc gttccgttac caactttaca catactttat atagctattc acttctatac
      661 actaaaaaac taagacaatt ttaattttgc tgcctgccat atttcaattt gttataaatt
      721 cctataattt atcctattag tagctaaaaa aagatgaatg tgaatcgaat cctaagagaa
      781 ttgagctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
      841 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      901 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      961 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1021 ttacgcgcag cgtgaccnnt acacttgcca gcgccctagc gcccgctcct ttcgcttttc
     1081 ttccnttcct ttctcgccac nnngccggnt ttccccgtcn annc
//
